Table 2. PCR parameter for molecular biological analysis

Primer name	Primer sequence (5’→3’)	Sense
LCO1490	GGTCAACAAATCATAAAGATATTGG	Forward
HCO2198	TAAACTTCAGGGTGACCAAAAAATCA	Reverse
TL2-J	ATGGCAGATTAGTGCAATGG	Forward
TK-N	GTTTAAGAGACCAGTACTTG	Reverse

Initial denaturation 94°C for 5 min; 35 cycles each of 94°C for 20 s, 55°C for 20 s, and 72°C for 50 s; and a final extension step at 72°C for 5 min.
PCR: polymerase chain reaction.