Gene | Primer name | Primer sequence (5′→3′) | Sense | References |
---|---|---|---|---|
Cytochrome oxidase subunit I ( | LCO1490 | GGTCAACAAATCATAAAGATATTGG | Forward | |
HCO2198 | TAAACTTCAGGGTGACCAAAAAATCA | Reverse | ||
Amplification parameter | Initial denaturation: 95°C for 5 min; 35 cycles: 95°C for 30 s, 50°C for 30 s, 72°C for 45 s; final extension: 72°C for 5 min |