Table 3. Reference sequences with the closest similarity to Eurycoma longifolia leaf samples in the study based on BLASTn analysis for trnL-trnF and ITS regions

No	Region	Similarity	Accession	% Similarity	% Query cover	Reference
1	trnL-trnF	Eurycoma apiculata	GU593014	100.00	100	Muellner-Riehl et al., 2016
		E. longifolia	MH751519	99.79	100	Ng et al., 2019
		Simarouba versicolor	MG599400	99.16	99	Devecchi et al., 2018
		Perriera madagascariensis	GU593020	98.96	100	Muellner-Riehl et al., 2016
		Homalolepis morettii	MG599405	98.96	100	Devecchi et al., 2018
2	ITS	E. longifolia	MN715379	98.16	83	Unpublished
		E. longifolia	MG643109	98.98	81	Devecchi et al., 2018
		Ailanthus altissima	OX327691	88.62	100	Unpublished
		Simaba guianensis	MG643102	93.19	81	Devecchi et al., 2018
		Homalolepis cuneata	MG643083	93.18	81	Devecchi et al., 2018

ITS: Internal transcribed spacer.