Table 2. Average diameter, filtration time, and WRV of DES-LCNF and Enz-LCNFs

Sample Enzyme concentration Sample (%) Enzymatic treatment time (h) Average diameter time (h) (nm) Filtration time (sec) WRV (%)
DES-LCNF - - 20.7 ± 4.0 111 1,558.0
Enz-LCNF 1 24 20.4 ± 3.3 111 1,496.4
3 12 18.6 ± 3.2 98 1,514.3
24 17.0 ± 3.1 66 1,465.3
48 16.6 ± 3.1 60 1,636.6
5 24 15.7 ± 2.6 65 1,395.9
WRV: water retention value, DES-LCNF: deep eutectic solvent-lignocellulose nanofibrils, Enz-LCNF: two-step DES pretreatment.